Image analysis in comparative genomic hybridization

Comparative Genomic Hybridization (CGH)

HSCT: Hematopoietic Stem Cell Transplantation W-CGH: Whole-Comparative Genomic Hybridization FISH: Fluorescent in situ Hybridization DIA: Digital Image Analysis

Whole-comparative genomic hybridization (W-CGH) allows one to identify copy number differences in highly repeated DNAs between two genomes. It allowed the identification of nuclear markers that can be used to distinguish cell populations from different individuals in a chimeric situation. We discuss the reliability of W-CGH accomplished with fluorescent in situ hybridization (FISH) and digital ...

Array comparative genomic hybridization in male infertility.

BACKGROUND Male infertility caused by a maturation arrest of spermatogenesis is a condition with an abrupt stop in spermatogenesis, mostly at the level of primary spermatocytes. The etiology remains largely unknown. METHODS We focused on patients with a complete arrest at the spermatocyte level (n = 9) and used array comparative genomic hybridization to screen for deletions or duplications th...

Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors.

Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two different fluorochromes. Regions of gain or loss of DNA sequences, such as deletions, duplicatio...

Genomic imbalances in childhood medulloblastoma by comparative genomic hybridization

We used comparative genomic hybridization (CGH) technique to identify the genetic changes in 12 childhood medulloblastoma samples obtained from archived paraffinembedded tissues belonging to children (0.7-15 years-old) using double step degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR). CGH analysis showed that the most common genetic alterations were gains of chromosomes 4p...